89ke Options
89ke Options
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receiver cells with the 89K-encoded GI-form T4SS transportation conduit. Up to now, whilst mobilization of genetic features by T4SSs has been effectively analyzed in Gram-detrimental bacteria, the transfer of Gram-optimistic mobile genetic components by T4SSs is presently restricted to conjugative plasmids and transposons.
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ORF, which encodes a hypothetical protein of unknown function. The direction of transfer is demonstrated by a horizontal arrow. The lessen portion of this determine displays the alignment with the 89K oriT
We offer genetic proof exhibiting that an 89K-borne integrase mediates site-precise excision and circularization of 89K, with contribution from an auxiliary protein named excisionase.
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To analyze the fundamental transfer system, we executed a systematic molecular dissection on the 89K PAI. Determined by our Earlier documented comparative sequence Examination, 89K is situated inside the genome of S. suis
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To find out the frequency of 89K excision, real-time qPCR assays utilizing SYBR Premix Ex Taq (TaKaRa) were being formulated to measure The share of cells in a very tradition that contained the 89K attP
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nick on Each and every of such precisely linearized fragments is proven in Fig. 5C. As predicted, a few distinct bands equivalent to the predicted sizes (based on nicking on the predicted oriT